Vaccinations with Dendritic Cells Can Elicit Preventive Immunity in a Squamous Cell Carcinoma Animal Model.
- Author:
Young Ik SON
1
;
Ye Jeung KO
;
Seung Won CHUNG
;
Chi Kyou LEE
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Samsung Medical Center, Sungkyunkwan University, Seoul, Korea. yison@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
Dendritic cells;
Vaccines;
Squamous cell carcinoma;
Immunotherapy;
Animal models
- MeSH:
Adoptive Transfer;
Animals*;
Antigens, Neoplasm;
Bone Marrow;
Carcinoma, Squamous Cell*;
Cell Line;
Dendritic Cells*;
Immunotherapy;
Mice;
Mice, Inbred C3H;
Models, Animal*;
T-Lymphocytes;
Vaccination*;
Vaccines
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2004;47(12):1289-1296
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Dendritic cells (DC) can effectively mediate the prevention and regression of a variety of solid malignancies when they are properly primed with tumor antigens. However, little has been determined about the efficacy of DCbased vaccine for the prevention of squamous cell carcinoma (SCC). SUBJECTS AND METHOD: Bone marrow derived DC of C3H mouse were pulsed with ultraviolet B-irradiated apoptotic SCCVII cells, which are known as a poorly immunogenic SCC cell line. After vaccinations with those DC, a tumorigenic dosage of healthy SCCVII cells were challenged into the flank of animals and the tumor growth was assessed in a blinded and coded fashion. RESULTS: DC pulsed with apoptotic SCCVII cells effectively prevented the formation of solid tumor in 81% of vaccinated animals and mediated significant retardation of tumor growth (p=0.0029) in 19% of vaccinated animals when compared to control groups. SCCVII cell-specific T-cell response (IFN-gamma production) was observed in the immunized mice. CONCLUSION: Adoptive transfer of DC primed with apoptotic SCCVII cells can serve as an effective preventive vaccine in the poorly immunogenic SCC animal model.
