Effects of Epigallocatechin Gallate on Adhesion, Invasion and Matrix Metalloproteinase Activity in MDA-MB-231 Human Breast Cancer Cells.
- Author:
Myung Hee BANG
1
;
Ji Hye KIM
;
Woo Kyoung KIM
Author Information
1. Department of Food Science and Nutrition, Dankook University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
EGCG;
MDA-MB-231;
breast cancer;
invasion;
matrix metalloproteinase
- MeSH:
Breast Neoplasms*;
Breast*;
Cell Adhesion;
Cell Movement;
Extracellular Matrix;
Fibronectins;
Gelatin;
Humans*;
Matrix Metalloproteinases;
Neoplasm Metastasis;
Peptide Hydrolases;
Tea
- From:The Korean Journal of Nutrition
2005;38(2):104-111
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Tumor invasion is composed of four steps: cell adhesion to the extracellular matrix, degradation of the extracellular matrix components, tumor cell motility followed by cell detachment. Matrix metalloproteinases (MMPs) are important proteinases that associated with degradation of matrix component. Epigallocatechin gallate (EGCG) is a major polyphenolic constituent of green tea. In the study, we examined the anti-invasive and MMP activity suppression effects of EGCG in MDA-MB-231 human breast cancer cells. MDA-MB-231 human breast cancer cells were cultured with various concentrations 0 - 100 micrometer of EGCG. EGCG significantly inhibited the cell adhesion to the fibronectin. Cell motility through gelatin filter and invasion to Matrigel were inhibited dose-dependently by EGCG treatment. EGCG also inhibited the activities of MMP-2, -9 and the amount of MMP-9 (alpha = 0.05). Therefore, EGCG may contribute to the potential beneficial food component to prevent the invasion and metastasis in breast cancer.
