The expression of Protein Kinase C Isozymes in the Rat Brain.
- Author:
Changmee KIM
1
;
Young Suk SUH
;
Hae Sun CHUNG
;
Seung Jun HWANG
;
Im Joo RHYU
;
Hyun KIM
Author Information
1. Dept. of Anatomy, Inst. of Human Genetics, College of Medicine, Korea University, Korea.
- Publication Type:Original Article
- Keywords:
PKC isozyme;
Rat brain;
Gene expression;
In situ hybridization histochemistry
- MeSH:
Animals;
Brain*;
Central Nervous System;
Cerebellum;
Gene Expression;
Hippocampus;
In Situ Hybridization;
Isoenzymes;
Neocortex;
Nervous System;
Olea;
Olfactory Bulb;
Olfactory Pathways;
Protein Kinase C*;
Protein Kinase C-epsilon;
Protein Kinases*;
Putamen;
Rats*;
RNA, Messenger;
Septal Nuclei;
Substantia Nigra;
Thalamus;
Tinea
- From:Korean Journal of Anatomy
1999;32(5):637-646
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The mRNA expression of protein kinase C (PKC) isozymes (alpha, beta, gamma, delta, epsilon and zeta) in the rat nervous system was investigated with in situ hybridization histochemistry. In the central nervous system of rat, each PKC isozyme mRNAs was expressed in isozyme-specific pattern. PKC alpha mRNA was highly expressed in the olfactory bulb, piriform cortex, hippocampus, substantia nigra compacta, and inferior olive. The expression of PKC beta was highest in the olfactory tubercle, piriform cortex, caudate putamen, accumbens nucleus, neocortex, hippocampus, basolateral amygdaloid nucleus, pontine nucleus, and cerebellum. PKC gamma mRNA was distributed in the caudate putamen, hippocampus and cerebellum and PKC delta was expressed in the thalamus. PKC epsilon had widespread distribution, with relatively high levels in the anterior olfactory nucleus, olfactory tubercle, tinea tecta, piriform cortex, dorsal lateral septal nucleus, neocortex, hippocampus and cerebellum. PKC zeta had widespread and low expression. The spacially differential expression of PKC isozymes (alpha, beta, gamma, delta, epsilon and zeta) suggests that each PKC isozyme may be related with specific cellular function in the nervous system.
