Induction of Apoptosis in Human Oral Squamous Carcinoma Cells by Extracellular Products from Pseudomonas aeruginosa.
- Author:
Gyoo Cheon KIM
1
;
Hyeun Hee KANG
;
Hyeon Cheol KIM
;
In Ryeon KIM
;
Moo Hyung LEE
;
Byung Chan KOO
;
Duk Han KIM
;
Ji Hak MIN
;
Bong Soo PARK
Author Information
1. Department of Oral Anatomy, College of Dentistry, Pusan National University, Korea. parkbs@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Psuedomonas aeruginosa;
Human oral carcinoma cells;
Apoptosis
- MeSH:
Animals;
Apoptosis*;
Bacteria;
Carcinoma, Squamous Cell*;
Caspase 3;
Caspase 6;
Cell Death;
Chromatin;
Humans*;
Lamin Type A;
Protein Synthesis Inhibitors;
Pseudomonas aeruginosa*;
Pseudomonas*
- From:Korean Journal of Anatomy
2005;38(4):337-344
- CountryRepublic of Korea
- Language:English
-
Abstract:
It was reported that cancer in humans and animals infected with microbial pathogens was regressed about 100 years ago. Bacteria are able to trigger apoptosis by a variety of mechanisms including the secretion of protein synthesis inhibitors, pore forming proteins, molecules activating the endogenous death machinery in the infected cell. This study was conducted in order to investigate whether extracellular products of Psuedomonas aeruginosa (EPPA) induce apoptosis in human oral carcinoma cells (OSC9). The EPPA showed cytotoxic effect on OSC9 cells in dose and time-dependent manner. The cell death was demonstrated to be due to apoptosis characterized by chromatin condensation and nuclear fragment. EPPA treatment induced cleavage of caspase-3 and caspase-6. The caspase substrates, PARP, DFF45 and lamin A were cleaved during EPPA-induced apoptosis. Taken together, EPPA induces apoptosis on human oral squamous carcinoma cells in caspase-dependent manner. Our data therefore provide that EPPA contains a novel antitumor agent for human oral squamous carcinoma.
