Comparative Study of BMP-2 Alone and Combined with VEGF Carried by Hydrogel for Maxillary Alveolar Bone Regeneration.
10.1007/s13770-015-0046-y
- Author:
Sook Kyoung KIM
1
;
Tae Hyung CHO
;
Jeong Joon HAN
;
In Sook KIM
;
Yongdoo PARK
;
Soon Jung HWANG
Author Information
1. Department of Biomedical Engineering, College of Medicine, Korea University, Seoul, Korea.
- Publication Type:Comparative Study ; Original Article
- Keywords:
Maxillary alveolar bone defect;
Bone regeneration;
Bone morphogenetic protein-2;
Vascular endothelial growth factor;
Synergistic effect
- MeSH:
Bone Density;
Bone Regeneration*;
Collagen;
Hydrogel*;
Intercellular Signaling Peptides and Proteins;
Molar;
Osteogenesis;
Vascular Endothelial Growth Factor A*;
von Willebrand Factor
- From:
Tissue Engineering and Regenerative Medicine
2016;13(2):171-181
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.
