Repair of Partial Thickness Cartilage Defects Using Cartilage Extracellular Matrix Membrane-Based Chondrocyte Delivery System in Human Ex Vivo Model.
10.1007/s13770-016-9043-z
- Author:
Do Young PARK
1
;
Byoung Hyun MIN
;
Hyun Jung LEE
;
Young Jick KIM
;
Byung Hyune CHOI
Author Information
1. Department of Anatomy, Ajou University School of Medicine, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
Partial thickness cartilage defect;
Cartilage extracellular matrix membrane;
Autologous chondrocyte implantation;
Cartilage repair
- MeSH:
Animals;
Cartilage*;
Cell Survival;
Chondrocytes*;
Extracellular Matrix*;
Humans*;
In Vitro Techniques;
Lifting;
Membranes;
Mice;
Mice, Nude
- From:
Tissue Engineering and Regenerative Medicine
2016;13(2):182-190
- CountryRepublic of Korea
- Language:English
-
Abstract:
Treatment options for partial thickness cartilage defects are limited. The purpose of this study was to evaluate the efficacy of the chondrocyte-seeded cartilage extracellular matrix membrane in repairing partial thickness cartilage defects. First, the potential of the membrane as an effective cell carrier was investigated. Secondly, we have applied the chondrocyte-seeded membrane in an ex vivo, partial thickness defect model to analyze its repair potential. After culture of chondrocytes on the membrane in vitro, cell viability assay, cell seeding yield calculation and cell transfer assay were done. Cell carrying ability of the membrane was also tested by seeding different densities of cells. Partial defects were created on human cartilage tissue explants. Cell-seeded membranes were applied using a modified autologous chondrocyte implantation technique on the defects and implanted subcutaneously in nude mice for 2 and 4 weeks. In vitro data showed cell viability and seeding yield comparable to standard culture dishes. Time dependent cell transfer from the membrane was observed. Membranes supported various densities of cells. Ex vivo data showed hyaline-like cartilage tissue repair, integrated on the defect by 4 weeks. Overall, chondrocyte-seeded cartilage extracellular membranes may be an effective and feasible treatment strategy for the repair of partial thickness cartilage defects.
