Expression of Epstein-Barr Virus in Inverted Papilloma by In Situ Hybridization and Polymerase Chain Reaction.
- Author:
Jung Soo KIM
1
;
Nam Jo PARK
;
June Sik PARK
;
Sang Sook LEE
;
Eun Ju SOHN
;
Seung Heon SHIN
Author Information
1. Department of Otolaryngology, College of Medicine, Kyungpook University, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
Inverted papilloma;
Polymerase chain reaction;
In situ hybridization;
Epstein-Barr virus
- MeSH:
Consensus;
Epithelium;
Genome;
Herpesvirus 4, Human*;
Humans;
In Situ Hybridization*;
Incidence;
Mucous Membrane;
Papilloma, Inverted*;
Paraffin;
Polymerase Chain Reaction*;
RNA, Messenger;
Turbinates
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1998;41(7):876-882
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The pathogenesis and etiology of inverted papilloma (IP) has not yet been clearly defined. The relationship between sinonasal IP and various strains of human papillomavirus (HPV) has been examined previously. Yet, there is little consensus regarding the incidence or role of HPV in IP. This study was performed to investigate the possible role of EBV in IP and to draw a relationship between histopathologic progression and EBV status. MATERIALS AND METHODS: This study is comprised of 19 cases of IP and 10 cases of turbinate mucosa as control. To find out the presence of EBV in paraffin block, we used PCR and ISH. Also, the author examined coexisting areas of dysplasia or malignant transformation and compared histologic findings with the results of molecular biologic studies. RESULTS: In PCR, the EBV genome was detected in 15 of 19 sinonasal IP (78.9%). By using ISH with the EBV oligonucleotide probe, EBV mRNA were found to be scattered throughout the epithelium in the IP with a similar incidence in PCR (13/19, 68.4.7%). One case of malignancy and all three dysplasia cases had EBV genome in PCR. In the middle turbinate mucosa which were used as control, 3/4 of the cases showed EBV genomes that are positive to PCR. CONCLUSION: The above results imply that EBV plays a role in the pathogenesis of IP and also indicate that the middle turbinate might be a site of viral persistence.
