Morphologic Change of Nasal Epithelium in Animal Model with Nasal Hyperreactivity.
- Author:
Weon Jo CHOI
1
;
Jin Uk JEONG
;
Woo Jin JEON
;
Ki Sang RHA
;
Chan Il PARK
Author Information
1. Department of Otolaryngology, College of Medicine, Chungnam National University, Taejon, Korea. otolar@chollian.net
- Publication Type:Original Article
- Keywords:
Nasal hyperreactivity;
TDI;
Light microscopy;
Electron microscopy
- MeSH:
Animals*;
Basement Membrane;
Cytoplasm;
Extracellular Space;
Guinea Pigs;
Microscopy, Electron;
Models, Animal*;
Nasal Mucosa*;
Rhinitis
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1998;41(7):883-891
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Nasal hyperreactivity is a cardinal pathophysiological feature in allergic or nonallergic rhinitis. However, the mechanisms underlying nasal hyperreactivity are largely unknown. One of the proposed mechanisms is that epithelial damage may contribute to the development of nasal hyperreactivity. However, it is hard to find studies on this topic. OBJECTIVE: The aim of this study is to demonstrate whether the morphological change of nasal epithelium exists in animal model with nasal hyperreactivity or not. MATERIALS AND METHODS: Guinea pigs were sensitized in 10% TDI-ethyl acetate solution for 5 days, after which a recess of 3 weeks were allowed before exposing them in 5% TDI-ethyl acetate solution twice a week for 4 weeks. Thereafter, we observed the morphologic change of nasal mucosa with a light microscope and an electron microscope. RESULTS: Under the light microscopic observation, there were no morphological changes including epithelial damage in the TDI-provoked group. Under the electron microscopic, however, the widening of the intercellular space, the disruption of the basement membrane and the cytoplasmic vacuolization were observed. CONCLUSION: Morphological changes of the nasal epithelium were found in the animal model with nasal hyperreactivity under electron microscopic observation. Further studies to verify the relationship between the morphological changes and the nasal hyperreactivity will be needed.
