Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages.
10.1007/s13770-017-0069-7
- Author:
Asieh HEIRANI-TABASI
1
;
Shirin TOOSI
;
Mahdi MIRAHMADI
;
Mohammad Amir MISHAN
;
Hamid Reza BIDKHORI
;
Ahmad Reza BAHRAMI
;
Javad BEHRAVAN
;
Hojjat NADERI-MESHKIN
Author Information
1. Department of stem Cells and Regenerative Medicine Research, ACECR-Khorasan Razavi Branch Institute, Mashhad 917751436, Iran. nahojjat@gmail.com
- Publication Type:Original Article
- Keywords:
Stem cells;
Reamer-irrigator-aspirator;
Iliac crest bone marrow;
Adipose tissue;
Chemokine receptors
- MeSH:
Adipose Tissue;
Bone Marrow;
Receptors, Chemokine*;
Stem Cells
- From:
Tissue Engineering and Regenerative Medicine
2017;14(5):605-615
- CountryRepublic of Korea
- Language:English
-
Abstract:
MSC-based therapy is providing a cure for degenerative diseases with unmet medical need and usually iliac crest bone marrow (ICBM) are being applied in clinics. Alternative sources, including adipose tissue and reamer/irrigator/ aspirator hold great potential for isolating MCSs. Here, we compared original MSCs features of adipose tissue (Ad-MSCs) and bone marrow of long-bone (RIA-MSCs) or iliac crest, and the expression of chemokine receptors (including CXCR4, CX3CR1, CXCR6, CXCR2, CCR1 and CCR7) in these three sources, which are important in the context of homing. We further investigated the role of SDF-1/CXCR4 axis as a key player in motility of different population of MSCs using Transwell migration assay. All cells exhibited typical MSCs characteristics. However, different MSCs sources expressed different levels of chemokine receptors. Generally, the expression of these chemokine receptors was decreased with increasing passage (P) number from 2 to 3. Interestingly, it was observed that the CXCR4 expression and migration capacity in Ad-MSCs is significantly higher than ICBM and RIA-MSCs in P2. Although our data showed that CXCR4 had highest expression in P2 Ad-MSCs, but it dramatically declined following sub-culturing in the P3. Hence, to improve homing of MSCs by means of chemokine/their receptors axis, the source of isolation and passage number should be considered for clinical applications.
