Apoptotic Neuronal Death in the Mouse Brain Induced by Experimental Japanese Encephalitis Virus Infection.
- Author:
Tae Hoon KANG
1
;
Soo Lim CHAE
;
Woo Young CHOI
;
Chan PARK
;
Jae Hwan NAM
;
Young Ran JOO
;
Keun Yong PARK
Author Information
1. Department of Anatomy, College of Medicine, The Catholic University of Korea, ibkimmd@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
JEV;
Immunohistochemistry;
TUNEL;
in vivo model;
Mouse
- MeSH:
Animals;
Apoptosis;
Asian Continental Ancestry Group*;
Brain Stem;
Brain*;
Cells, Cultured;
Cerebellum;
Cerebral Cortex;
Diencephalon;
DNA;
DNA Fragmentation;
Encephalitis;
Encephalitis Virus, Japanese*;
Encephalitis, Japanese*;
Hippocampus;
Humans;
Immunohistochemistry;
In Situ Nick-End Labeling;
Mice*;
Neurons*;
Telencephalon
- From:Korean Journal of Anatomy
2005;38(3):259-264
- CountryRepublic of Korea
- Language:English
-
Abstract:
Japanese encephalitis virus (JEV)may cause acute encephalitis in humans and induce severe cytopathic effects in various types of cultured cells. To investigate whether JEV infection induces apoptosis, we examined DNA fragmentation and apoptosis in the specific region of the JEV infected mouse brain by DNA oligonucleosomal laddering and in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)technique and immunohistochemical study. JEV infections in the mouse brain were detected in the telencephalon, the diencephalons, and the brain stem, but not in the cerebellum and the hippocampus. Fragmentation of cellular DNA into oligonucleosome-length ladders was only observed in tissue samples prepared from the cerebral cortex. In addition, the large number of TUNEL-positive cells was observed in the cerebral cortex. Double-labeling experiment with TUNEL staining and immunostaining for the JEV showed that TUNEL-positive neurons containing JEV immunoreactivity. These results suggest that JEV infection may evoke apoptotic neuronal death in the mouse brain, which plays an important role in the pathogenesis of Japanese encephalitis.
