Expression of Senescence-related Proteins in Rat Fracture Callus.
- Author:
Sang Ho SONG
1
;
Young Euy PARK
;
Young Hee CHOI
;
Chang Gu SHIM
;
Seong Woo CHO
Author Information
1. Department of Orthopaedic Surgery, Inchon Christian Hospital, Inchon, Korea.
- Publication Type:Original Article
- Keywords:
Senescence-related protein;
Callus;
Immunohistochemistry;
Aging;
p16;
p21;
c-fos;
c-jun
- MeSH:
Aging;
Animals;
Antibodies;
Bone and Bones;
Bony Callus*;
Chondrocytes;
Femur;
Genes, jun;
Immunohistochemistry;
Rats*;
Rats, Sprague-Dawley
- From:The Journal of the Korean Orthopaedic Association
2001;36(5):479-484
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the expression of senescence-related proteins according to the aging process and to determine the role of senescence-related proteins in the bone tissue and their effects on the process of bone union. MATERIALS AND METHODS: 18 Sprague-Dawley rats (8 weeks old: 7, 32 weeks old: 6, and 70 weeks old: 5) were used in the experiment. A unilateral closed femur fracture was made, and the fracture callus was obtained 2 weeks after the fracture. The ossification process was observed in proliferative chondrocytes, the hypertrophic chondrocytes, and in the mesenchymal layer individually by immunohistochemistry, using p16, p21, c-fos and c-jun antibodies. RESULTS: There was no significant differences in the manifestation of p-16, p-21, c-fos, c-jun gene according to the age. The positive ratio of p-16 was maximal in proliferative chondrocytes (54.93%) and decreased in the mesenchymal layer (46.48%), and in hypertrophic chondrocytes (10.85%), in order. The positive ratio of c-fos was maximal in proliferative chondrocytes (73.32%) and decreased in the mesenchymal layer (51.84%), and in hypertrophic chondrocytes (9.64%), in order. CONCLUSION: We believe that senescent genes in the bone tissue participate in the differentiation of osteochondral cells and in the process of fracture callus ossification.
