The Effect of Iron Depletion on the Monocytic Adhesion, Differentiation and SR-A Expression.
- Author:
Geom Seog SEO
1
;
Suck Chei CHOI
;
Eun Young CHOI
;
Tae Hyeon KIM
;
Yong Ho NAH
;
Jae Min OH
;
Sang Wook KIM
;
Chang Duk JUN
Author Information
1. Department of Gastroenterology, Wonkwang University, School of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Iron deficiency;
Host immune response;
Desferrioxamine;
Monocyte;
Macrophage;
Cell differentiation
- MeSH:
Azure Stains;
Cell Adhesion;
Cell Differentiation;
Chronic Disease;
Citric Acid;
Deferoxamine;
Homeostasis;
Homicide;
Hydroxyl Radical;
Iron*;
Macrophages;
Monocytes;
Phagocytosis;
Polystyrenes
- From:Korean Journal of Anatomy
2004;37(6):557-563
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Maintenance of cellular iron homeostasis is a prerequisite for proliferation and differentiation of cells, and is also a central role in the regulation of immune function. Monocyte-macrophages play an important roles in host defense, particularly in the inflammatory process of acute and chronic disease. The reason that an iron is important in these cell is because an iron is indispensable in a generation of hydroxyl radical for bacterium killing. Because of the role of iron in the monocytic THP-1 cell differentiation is not become clear, we investigated whether THP-1 cell can differentiate to macrophage-like cell using of iron and iron chelator which cause iron depletion. The cell differentiation was not able to observe by iron treatment, by the way, the cell adhesion was increased in DFO treated monocyte and cellular pseodopodial extension, change of a nucleus-cytoplasmic ratio were showed in Differential interference contrast (DIC) and Giemsa staining, and it was inhibited by ferric citrate (FC). Increased polystyrene bead phagocytosis by DFO treatment of THP-1 cell were detected through FACS and rhodamine-phallodin staining. The SR-A expression, which was a cell differentiation marker, was increased by DFO treatment of THP-1 cell. These results suggest that iron depletion by DFO can promote THP-1 cell diffentiation into macrophage-like cell, and this may carrying out important role in the immune response.
