Zinc translocation and heat shock protein induction in rat brains following kainate seizures.
- Author:
Donghou KIM
1
;
Jong Hwan LEE
;
Hea Nam HONG
;
Jae Young KOH
Author Information
1. Department of Anatomy, and 1neurology, College of Medicine, University of Ulsan, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Kainate;
Neuronal death;
Zinc;
HSP72
- MeSH:
Amygdala;
Animals;
Brain*;
Heat-Shock Proteins*;
Hippocampus;
Hot Temperature*;
Immunohistochemistry;
Kainic Acid*;
Limit of Detection;
Neurons;
Rats*;
Seizures*;
Zinc*
- From:Korean Journal of Anatomy
1999;32(4):489-496
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Translocation of synaptic zinc may mediate neuronal death in pathological conditions. In this study, we examined the possible correlation between zinc translocation and heat shock protein (HSP)72 induction in rat brains following kainate seizures. Zinc accumulation, visualized by Timm's method, occurred in degenerating neurons in hippocampus, amygdala, and cortex 6~24 h after kainate injection. Immunohistochemistry with anti-HSP72 antibody revealed HSP induction largely in areas where zinc accumulation occurred. At the cellular level, however, most HSP72 immunoreac-tive neurons were found to be Timm (-) and morphologically intact. Present results suggest that intense zinc translocation may induce neuronal death before possible HSP induction. However, we could not rule out the possibility that sublethal zinc translocation, below the detection limit by Timm's method, may play a role in HSP72 induction.
