Immunohistochemical Study of Insulin-Like Growth Factor-I (IGF-I) in the Olivocerebellar System of Developing and Adult Rats.
- Author:
In Youb CHANG
1
;
Sang Pil YOON
;
Yoon Young CHUNG
;
Jong Joong KIM
;
Jeong Seok MOON
;
Young Taek KIM
;
Young Hune CHANG
Author Information
1. Department of Anatomy, College of Medicine, Chosun University, Korea.
- Publication Type:Original Article
- Keywords:
Climbing fiber;
Purkinje cell;
IGF-I;
Olivocerebellar projection;
Development
- MeSH:
Adult*;
Animals;
Cerebellum;
Humans;
Insulin-Like Growth Factor I;
Neurons;
Olea;
Parturition;
Purkinje Cells;
Rats*
- From:Korean Journal of Anatomy
1999;32(4):509-516
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The developmental topography of olivocerebellar projection is not fully understood. Insulin-like growth factor-I (IGF-I) plays important roles in neural development. This study examined to observe IGF-I-like immunoreactivity (IGF-I IR) in the cerebellum and inferior olive of postnatal developing and adult rats. IGF-I immunoreactive Purkinje cells exhibited spatially and temporally regulated distribution which correlates with climbing fiber development. At birth a few IGF-I immunoreactive Purkinje cells were stained weakly only in the ventral vermis. By P7, all Purkinje cells of the vermis and hemispheres were positively labelled. A subpopulation of Purkinje cells lost IGF-I IR, and IGF-I IR Purkinje cells were divided into discrete population arranged in sagittal strips which were separated by non-reactive Purkinje cells. In the inferior olive, neurons showed IGF-I IR between P0 and P7. By P10 the inferior olive neurons were all negative for IGF-IR and this was mantained to adulthood. The IGF-I IR for Purkinje cell and inferior olive coincides with climbing fiber development and thus the results of this study support the hypothesis that IGF-I is specially involved in the refinement of olivocerebellar topography during synaptogenesis.
