Expression of Midkine mRNA in Human Nasal Mucosa.
- Author:
Jeong Joon KIM
1
;
Sung Bae CHOI
;
Chang Haeng LEE
;
Ki Jung LIM
;
Byung Hoon OH
;
Eun Joong KIM
;
Heung Man LEE
;
Sang hag LEE
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Korea University, Seoul, Korea. sanghag@ns.kumc.or.kr
- Publication Type:Original Article
- Keywords:
Midkine;
RT-PCR;
In situ hybridization;
Inferior turbinate;
Nasal polyp
- MeSH:
Blotting, Southern;
Cytokines;
Endothelium, Vascular;
Epithelium;
Humans*;
In Situ Hybridization;
Nasal Mucosa*;
Nasal Polyps;
Physiology;
Polymerase Chain Reaction;
Polyps;
Rhinoplasty;
RNA;
RNA, Messenger*;
Turbinates
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2002;45(4):335-340
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: A heparin-binding polypeptide called midkine is a family of secreted growth/differentiation cytokines and has a role in tumor growth by enhancing endothelial proliferation, vascular density and angiogenesis. In this respect, midkine may be involved in the pathogenesis of nasal polyposis. The aim of the present study is to evaluate the expression of midkine mRNA in the human nasal mucosa and polyps. MATERIALS AND METHOD: The total RNA was isolated from freshly disected inferior turbinate of patients who underwent rhinoplasty and from nasal polyps of chronic rhinosinusitis patients. The expression and distribution of midkine mRNA was investigated by reverse transcriptse- polymerase chain reaction (RT-PCR) and in situ hybridization. The midkine mRNA expression in nasal mucosa and polyps were semi-quantitatively evaluated by Southern blot hybridization. RESULTS: The expression of midkine mRNA was identified in both normal inferior turbinate and nasal polyp. Histochemistry of in situ hybridization revealed that midkine mRNA in normal inferior turbinate was intensely expressed in the surface epithelium, submucosal glands, vascular endothelium, and inflammatory cells scattered in submucosal tissues. Midkine mRNA was expressed in the nasal polyps, many inflammatory cells and newly formed vascular endothelium, but not in the newly formed glandular epithelium. In semi-quantitative southern blot hybridization, midkine mRNAs did not have different expression levels between inferior turbinate and nasal polyps. CONCLUSION: These results indicate that midkine mRNA is innately expressed in human nasal mucosa, playing a role in nasal physiology. Also, the results show that midkine may be involved in the pathogenesis of nasal polyps via angiogenesis, tissue growth, and inflammatory process.
