In vivo Mapping of the Cis-acting Element Responsible for Spatial Regulation of the EphA8 Gene Expression in Mesencephalon.
- Author:
Jieun KIM
1
;
Soochul PARK
Author Information
1. Department of Biological Science, Sookmyung Women's University, Korea. scpark@sookmyung.ac.kr
- Publication Type:Original Article
- Keywords:
EphA8;
Ephrin;
Midbrain;
Enhancer;
Transgenic mice
- MeSH:
Animals;
beta-Globins;
DNA;
Gene Expression*;
Humans;
Mesencephalon*;
Mice;
Mice, Transgenic
- From:Korean Journal of Anatomy
2005;38(2):125-131
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was aimed to map the 1 kb cis-acting DNA of ephA8 gene, which regulates the spatial and temporal expression in the anterior mesencephalon. It was demonstrated that the 1 kb ephA8 enhancer DNA was sufficient to drive the lacZ expression to the anterior mesencephalon under the human beta-globin minimal promoter. It was also found that a 180 bp within the 1 kb enhancer DNA was highly conserved between human and mouse, and that this 180 bp DNA was capable of inducing the lacZ expression in the anterior mesencephalon under the ephA8 or human beta-globin basal promoter. Further analysis using 5'-55 bp deleted or 3'-55 bp deleted mutant DNA revealed that the 55 bp portion present at the 3'-end of 180 bp DNA was critical for the regulation of ephA8 gene expression in the anterior mesencephalon. Taken together, these results indicate that 180 bp ephA8 enhancer DNA contains cisacting elements for the regulation of ephA8 gene expression in the anterior mesencephalon.
