The Effect of Substance P on Osteoclastogenesis and Osteoclastic Bone Resorption in vitro.
- Author:
Soo Joon SOHN
1
Author Information
1. Department of Otolaryngology, Catholic University of Daegu School of Medicine, Daegu, Korea. sjsohn@cu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Osteoclasts;
Substance P;
Receptors;
Neurokinin-2;
Bone resorption
- MeSH:
Animals;
Bone Resorption*;
Cholesteatoma;
Dentin;
Mice;
NF-kappa B;
Osteoclasts*;
Receptors, Neurokinin-2;
RNA, Messenger;
Substance P*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2003;46(10):822-826
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Osteoclasts are the principal cell of bone resorption playing a major role in focal bone erosion associated with cholesteatoma. This study was conducted in order to investigate direct effect of substance P (SP) on osteoclastogenesis and osteoclastic bone resorption in vitro. MATERIALS AND METHOD: SP dose response was measured in receptor activator for NF-kappaB ligand (RANKL)-induced mouse osteoclast culture and osteoclastic bone resorption assay. RT-PCR was performed for the expression of neurokinin(NK) receptor mRNAs in osteoclasts. RESULTS: Treatment with SP (100 nM and 1000 nM) significantly increased osteoclastogenesis. SP (0.1 nM and 1 nM) significantly increased resorption surface area on dentin slices by osteoclasts. Cultured osteoclasts expressed NK-2 receptor mRNA. CONCLUSION: SP has a direct upregulatory effect on osteoclastogenesis and osteoclastic bone resorption that is mediated possibly by NK-2 receptor.
