Osteoinduction Using Recombinant Bone Morphogenetic Protein-7 Gene.
10.4055/jkoa.2004.39.6.598
- Author:
Woo Seok JANG
1
;
Jeong Rae KIM
;
Wook Jin SOHN
;
Jae Sung SEO
;
Myun Whan AHN
;
Yeon Sil JANG
;
Hyun Kyung KIM
;
Jae Ryong KIM
Author Information
1. Department of Orthopaedic Surgery, College of Medicine, Yeungnam University, Daegu, Korea. mwahn@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
BMP7;
Adenovirus;
Osteogenesis;
Gene therapy
- MeSH:
Adenoviridae;
Alkaline Phosphatase;
Animals;
Bone Diseases;
Cell Line;
Clone Cells;
DNA, Complementary;
Fibroblasts;
Genetic Therapy;
HEK293 Cells;
Humans;
Mice;
Muscles;
Musculoskeletal Diseases;
Osteogenesis;
Osteosarcoma;
Plasmids;
Regeneration;
Subcutaneous Tissue
- From:The Journal of the Korean Orthopaedic Association
2004;39(6):598-606
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aim of this study was to develop recombinant human Bone Morphogenetic Protein-7 (BMP7)-stable cells and recombinant human BMP7 adenoviruses (AdBMP7) for osteoinduction and osteoregeneration in musculoskeletal diseases. MATERIALS AND METHODS: The human BMP7 cDNA was amplified from a human osteosarcoma cell line, U2OS using a reverse transcription-polymerase chain reaction and cloned into a eukaryotic expression vector. The BMP7-stable HEK293 cells (HEK293/BMP7) were prepared by transfecting the recombinant BMP7 plasmid vector. The recombinant human BMP7 adenovirus (AdBMP7) was constructed using the AdEasy vector system. The BMP7 expression levels in HEK293/BMP7 and AdBMP7 were measured by activity staining for alkaline phosphatase in mouse C2C12 promyoblast cells. The BMP7-stable HEK293 cells, AdBMP7 itself, or AdBMP7-transduced human fibroblasts were injected into the subcutaneous tissues and the calf muscles of immunocompromised mice. The amount of ectopic bone formation was evaluated by radiographic and histological analyses. RESULTS: Ectopic bone formation was observed after injecting the BMP7-stable HEK293 cells with either the AdBMP7 itself or AdBMP7-transduced human fibroblasts into the subcutaneous tissues and calf muscles of immunocompromised mice. CONCLUSION: These results showed that HEK293/BMP7 cells and AdBMP7 have a significant potential for bone formation and the regeneration of various bone diseases.
