Effect of Rhodiola Sachalinensis Administration and Endurance Exercise on Insulin Sensitivity and Expression of Proteins Related with Glucose Transport in Skeletal Muscle of Obese Zucker Rat.
- Author:
Jae Keun OH
1
;
Young Oh SHIN
;
Hee Jung JUNG
;
Jung Eun LEE
Author Information
1. Department of Sports Medicine, Korea National Sport University, Seoul, Korea. ojk8688@hanmail.net
- Publication Type:Original Article
- Keywords:
Rhodiola sachalinensis;
exercise;
obesity;
insulin resistance;
homa;
skeletal muscle;
glut4;
snare protein;
zucker rat
- MeSH:
Animals;
Body Weight;
Cell Membrane;
Glucose Transporter Type 4;
Glucose*;
Insulin Resistance*;
Insulin*;
Muscle, Skeletal*;
Negotiating;
Obesity;
Oxidoreductases;
Rats*;
Rats, Zucker;
Rhodiola*;
Running;
SNARE Proteins;
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins;
Vesicle-Associated Membrane Protein 2
- From:The Korean Journal of Nutrition
2006;39(4):323-330
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Peripheral insulin resistance in obese/type II diabetes animals results from an impairment of insulin-stimulated glucose uptake into skeletal muscle. Insulin stimulate the translocation of GLUT4 from intracellular location to the plasma membrane. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) is implicated in mediation of fusion of GLUT4-containing vesicle with the plasma membrane. Present study investigated regulatory effects of Rhodiola sachalinensis administration and exercise training on the expression of GLUT4 protein and SNAREs protein in skeletal muscles of obese Zucker rats. Experimental animals were randomly assigned into one of five groups ; lean control (LN), obese control (OB), exercise-treated (EXE), Rhodiola sachalinensis-treated (Rho), combine of Rho & EXE (Rho-EXE). All animals of exercise training (EXE, Rho-EXE) performed treadmill running for 8 weeks, and animals of Rho groups (Rho, Rho-EXE) were dosed daily by gastric gavage during the same period. After experiment, blood were taken for analyses of glucose, insulin, and lipids levels. Mitochondrial oxidative enzyme (citrate synthase, CS ; beta-hydroxyacyl-CoA dehydrogenase, beta-HAD) activity were analysed. Skeletal muscles were dissected out for analyses of proteins (GLUT4, VAMP2, syntaxin4, SNAP23). Results are as follows. Exercise and/or Rhodiola sachalinensis administration significantly reduced body weight and improved blood lipids (TG, FFA), and increased insulin sensitivity. Endurance exercise significantly increased the activity of mitochondrial enzymes and the expression of GLUT4 protein, however, administration of Rhodiola sachalinensis did not affect them. The effect of exercise and/or Rhodiola sachalinensis administration on the expression of SNARE proteins was unclear. Our study suggested that improvement insulin sensitivity by exercise and/or Rhodiola sachalinensis administration in obese Zucker rats is independent of expression of SNARE proteins.
