Expansion and New Creation of Human Septal Cartilage from Biopsied Fragment: Using in vivo 3-D Culture of Chondrocytes.
- Author:
Dong Joon PARK
1
;
Seog In PAIK
;
Jeong Pyo BONG
;
Sang Yoo PARK
;
Gi Won YU
;
Jeung Gweon LEE
Author Information
1. Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, Wonju, Korea.
- Publication Type:Original Article
- Keywords:
Chondrocytes;
Cell culture;
Cartilage;
Alginate
- MeSH:
Animals;
Calcium;
Cartilage*;
Cell Culture Techniques;
Chondrocytes*;
Culture Techniques;
Humans*;
Hyaline Cartilage;
Mice;
Mice, Nude;
Subcutaneous Tissue;
Tissue Donors
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1997;40(9):1274-1279
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The use of autogenous cartilage has the problem of donor site morbidity and limited supply in reconstructive surgery. OBJECTIVES: The purpose of this study is to increase the size and the volume of the autogenous small cartilage by the new three dimensional culture technique. MATERIALS AND METHODS: The chondrocytes from biopsied human septal cartilage were subcultured. The increased cells were encapsulated with calcium alginate and were injected to the subcutaneous tissue of the athymic nude mouse for in vivo 3-dimensional culture. RESULTS: After 8 weeks of the injection, the implanted subcutaneous nodule was seemed to be the natural cartilage and the histoarchtectures revealed characteristics of the hyaline cartilage. CONCLUSION: This results suggest that the small biopsied cartilage can be expanded by the techniques of subculture and the 3-dimensional culture., and can potentially overcome the limited availability of autogenous donor cartilage.
