Expression Patterns of Erythropoietin and Erythropoietin Receptor in the Spiral Ganglion of Guinea Pig after Noise Exposure.
- Author:
Dong Hyun KIM
1
;
Jae Yong PARK
;
Ah Young KIM
;
Jin Woong CHOI
;
Wook Kyoung HAN
;
Yong Ho PARK
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Chungnam National University College of Medicine, Daejeon, Korea. parkyh@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Noise;
Spiral ganglion;
Erythropoietin;
Erythropoietin receptor
- MeSH:
Animals;
Cochlea;
Ear, Inner;
Erythropoiesis;
Erythropoietin;
Guinea;
Guinea Pigs;
Hearing;
Kidney;
Noise;
Phalloidine;
Receptors, Erythropoietin;
Spiral Ganglion
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2008;51(1):16-21
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Erythropoietin (EPO) is produced in the kidney and locally in the CNS and acts through binding to erythropoietin receptor (EPO-R). Apart from playing an essential role in erythropoiesis, recent research has shown that EPO has neurotrophic and neuroprotective functions in the CNS and found EPO and EPO-R in the inner ear. The aim of this study is to investigate distribution and expression of EPO and EPO-R in the inner ear after noise exposure. MATERIALS AND METHOD: Normal guinea pigs were exposed to noise. Ten of them were sacrificed at 1 hour of the noise exposure (group B) and another 10 animals were sacrificed at day 7 (group C). Four were normal controls that were not exposed to noise (Group A). Auditory function was evaluated by ABR for 7 days. Noise-induced morphological changes of cochlea were studied by phalloidin stain. The expression of EPO and EPO-R was examined by immunofluorescence. RESULTS: The hearing threshold shift reached a level of 40 dB SPL at 8 kHz at day 1 after noise exposure and underwent a partial recovery at day 7. Increased expression of EPO and EPO-R were observed at the level of spiral ganglion cells in the noise-exposed animals. CONCLUSION: It is suggested that noise exposure affects the distribution and expression of EPO and EPO-R in the inner ear.
