Effects of several cytokines on the functions of fetal rat osteoblast-like cells in vitro.
- Author:
Hee Sung HAN
;
Jung Keun KIM
;
Young Il CHANG
- Publication Type:In Vitro ; Original Article
- Keywords:
Osteoblast-like cell;
Interleukin-1beta;
Tumor necrosis factor alpha;
Interferon gamma;
Phosphatase;
Bone remodeling
- MeSH:
Acid Phosphatase;
Alkaline Phosphatase;
Animals;
Bone Remodeling;
Cytokines*;
Interleukin-1beta;
Rats*;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Orthodontics
1995;25(6):689-696
- CountryRepublic of Korea
- Language:English
-
Abstract:
Effects of several cytokines(IL -1beta, TNFalpha, and IFNgamma) have been examined on fetal rat osteoblast-like cells. To investigate whether cytokines play direct causal roles in production of lysosomal enzyme, fetal rat osteoblast-like cells were treated with IL-1beta, TNFalpha, and IFNgamma, respectively or combined. And acid phosphatase was determined by biochemical method. Alkaline phosphatase was assayed to determine the effects of IL-1beta, TNFalpha, and IFNgamma on the expression of this enzyme. And also experiment of calcified nodule formation was performed to assess the effects of cytokines on the bone-forming activity of osteoblast-like cells in vitro. Acid phosphatase activity was significantly increased by the addition of IL-1beta and TNFalpha, whereas decreased by IFNgamma, However, no significant changes in alkaline phosphatase activity was observed when the osteoblast-like cells were treated with IL-1beta and TNFalpha. Interestingly, IFNalpha showed stimulatory effect on alkaline phosphatase activity. The number of calcified nodules was decreased by treatment of cultures with 1ng/ml IL -lbeta, 20ng/ml TNFalpha, and 500 u/ml IFNgamma continuously for 21 days, while considerable number of calcified nodules were formed in control group of osteoblast-like cell in culture for 21 days. These results seem to suggest that cytokines may play crucial roles in bone remodeling through the direct action on the osteoblast-like cell.
