The Effect of Topical Steroid Nasal Instillation in Induced Anosmic Mice.
- Author:
Byung Guk KIM
1
;
So Young PARK
;
Dae Gun JUNG
;
Jin Hee CHO
;
Dong Mok LEE
;
Myung Won KIM
Author Information
1. Department of Otolaryngology-HNS, The Catholic University of Korea College of Medicine, Seoul, Korea. coolkim@chol.com
- Publication Type:Original Article
- Keywords:
Anosmia;
Olfaction;
Zinc sulfate;
Olfactory receptor neuron
- MeSH:
Animals;
Immune Sera;
Immunohistochemistry;
Mice*;
Nasal Cavity;
Olfaction Disorders;
Olfactory Marker Protein;
Olfactory Mucosa;
Olfactory Receptor Neurons;
Regeneration;
Smell;
Zinc Sulfate;
Mometasone Furoate
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2004;47(12):1256-1260
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The purpose of this study was to evaluate the effect of intranasal Mometasone furoate instillation into the nasal cavity of mice which had peripherally induced anosmia. SUBJECTS AND METHOD: Three groups of mice were studied: normal control group (nasal instillation of normal saline, n=6), Mometasone furoate non-instillation group (no treatment after nasal instillation of zinc sulfate, n=12), and Mometasone furoate instillation group (daily mometasone furoate instillation after nasal instillation of zinc sulfate, n=12). Tissues of olfactory mucosa were obtained on 1, 2, 3, 4 weeks after the instillation of zinc sulfate, and processed for immunohistochemistry using antisera to olfactory marker protein (OMP) for evaluation of olfactory regeneration. RESULTS: No OMP-positive cells were observed in the first week after the instillation of zinc sulfate in both groups. However, OMP-positive cells began appearing in the second week in both groups and gradually increased as time goes by. In the Mometasone furoate instillation group, the increase of OMP-positive cells was significantly greater than that of Mometasone furoate non-instillation group. CONCLUSION: Mometasone furoate instillation enhances regeneration of olfactory receptor cells after injury. Mometasone furoate instillation can be suggested as an effective treatment modality for olfactory dysfunction.
