The Change of Molecular Event of p53 by Cisplatin and 5-Fluorouracil in Hypopharyngeal Cell Line(PNUH-12).
- Author:
Kyong Myong CHON
1
;
Byung Joo LEE
;
II Woo LEE
;
Young Il MOON
;
Hwan Jung ROH
;
Soo Geun WANG
;
Eui Kyung GOH
;
So Rin KIM
;
Eun Yup LEE
Author Information
1. Department of Otolaryngology, College of Medicine, Busan National University, Busan, Korea. voicelee@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Cisplatin;
Fluorouracil;
Hypopharyngeal neoplasms
- MeSH:
Apoptosis;
Blotting, Western;
Cell Cycle Checkpoints;
Cell Line;
Cisplatin*;
DNA;
DNA Fragmentation;
Exons;
Flow Cytometry;
Fluorouracil*;
G1 Phase;
Head and Neck Neoplasms;
Hypopharyngeal Neoplasms;
Point Mutation;
S Phase
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2002;45(10):984-989
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES AND BACKGROUND: In head and neck cancer including hypopharyngeal carcinoma, cisplatin and 5-fluorouracil usually have been used as neoadjuvant chemotherapeutic agents. We investigated the difference in the influences of cisplatin and 5-fluorouracil (5-FU) on the p53 protein expression and cell responses (cell cycle arrest and apoptosis) in the hypopharyngeal cell line (PHUH-12). METHOD: PNUH-12 with a mutant type p53 (one point mutation at the 78th base, C to G, in exon 7) was treated with cisplatin and 5-FU. Changes in the cell line were assessed by MTT assay, Western blotting (p53 and p21 protein), DNA fragmentation, PI stain, and DNA flow cytometry. RESULTS: The p53 protein expression was increased after the treatment with cisplatin and 5-FU. The expression of p21 protein was increased after the treatment with 5-FU, not cisplatin. With cisplatin, we observed apoptosis by DNA fragmentation and PI stain and the increased S phase on DNA flow cytometry. But, with 5-FU, we couldn't observe apoptosis by DNA fragmentation, PI, and flow cytometry and only the increased G1 phase on DNA flow cytometry. CONCLUSION: In hypopharyngeal cell line (PNUH-12), cisplatin induced p53 dependent apoptosis and 5-FU induced p53 and p21 dependent G0/G1 cell cycle arrest, but not apoptosis.
