Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells.
- Author:
Young Chang LIM
1
;
Ki Woong CHO
;
Hak Cheol KWON
;
Sung Un KANG
;
Jung Hee PYUN
;
Mi Hye LEE
;
Hye Sook HWANG
;
Jang Hee KIM
;
Ha Neul LEE
;
Eun Chang CHOI
;
Chul Ho KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Apoptosis; Bacillus; Marine toxins; Head and neck cancer; Cytotoxicity
- MeSH: Animals; Apoptosis; Bacillus; Caspase 3; Cell Line; Cell Survival; Cytochromes c; Drug Toxicity; Embryonic Structures; Head; Head and Neck Neoplasms; Humans; KB Cells; Marine Toxins; Mice; Signal Transduction; Toxicity Tests; Transplantation, Heterologous; Zebrafish
- From:Clinical and Experimental Otorhinolaryngology 2010;3(4):217-225
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: To determine whether a novel marine micro-organism with anticancer properties, H31, the metabolic product of Bacillus SW31, has anti-tumor effects on head and neck cancer, and potential for apoptotic-enhancing anti-cancer treatment of affected patients. METHODS: The cell viability and apoptosis assays were performed. Changes in the signal pathway related to apoptosis were investigated. Then, the therapeutic effects of H31 were explored in mouse xenograft model and drug toxicity of H31 was examined in zebrafish model. RESULTS: We identified the anticancer activity of H31, a novel metabolic product of Bacillus SW31. Bacillus SW31, a new marine micro-organism, has 70% homology with Bacillus firmus and contains potent cytotoxic bioactivity in head and neck cancer cells using MTT assay. Combined with c-JUN, p53, cytochrome C, and caspase-3, H31 induced apoptosis of KB cells, a head and neck cancer cell line. In a separate in vivo model, tumor growth in C3H/HeJ syngeneic mice was suppressed by H31. In addition, in a zebrafish model used for toxicity testing, a considerable dose of H31 did not result in embryo or neurotoxicity. CONCLUSION: Growth inhibition and apoptosis were achieved both in vitro and in vivo in head and neck cancer cells after exposure to H31, a metabolite from the marine Bacillus species, without any significant toxicity effects even at considerable H31 dose concentrations.
