Effects of Dexamethasone on RANTES Expression of Nasal Fibroblast.
- Author:
Young Wan JIN
1
;
Joong Seang CHO
;
Hoon KIM
;
Chun Dong KIM
;
Soung Yong JIN
;
Chang Il CHA
Author Information
1. Department of Otolaryngology, Korea Veterans Hospital, Seoul, Korea. ywjin@mdhouse.com
- Publication Type:Original Article
- Keywords:
Fibroblast;
RANTES;
Dexamethasone
- MeSH:
Chemokine CCL5*;
Cytokines;
Dexamethasone*;
Enzyme-Linked Immunosorbent Assay;
Fibroblasts*;
Granulocyte-Macrophage Colony-Stimulating Factor;
Humans;
Interleukin-8;
Population Characteristics;
Tumor Necrosis Factor-alpha;
Turbinates
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2001;44(9):930-935
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Fibroblasts play an indirectly augmenting effector role in allergic inflammatory response by releasing different proinflammatory cytokines, including RANTES, GM-CSF, IL-8 after stimulation by other inflammatory cytokines such as IFN-gamma, TNF-alpha. The aim of this study was to investigate expression of RANTES in allergic and non-allergic nasal fibroblasts after stimulation with IFN-gamma and TNF-alpha, and to study the effect of dexamethasone on the RANTES expression of nasal fibroblast cell. MATERIALS AND METHODS: Using the 3rd passage of fibroblasts taken from the inferior turbinates of allergic and non-allergic patients, we evaluated the RANTES expression of fibroblasts after the IFN-gamma, TNF-alpha stimulation in the presence or in the absence of dexamethasone by ELISA. RESULTS: The expression of RANTES in allergic nasal fibroblasts stimulated by cytokines was stronger than in non-allergic nasal fibroblasts stimulated by cytokines. And dexamethasone suppressed the RANTES expression in allergic nasal fibroblasts stimulated by IFN-gamma. However, dexamethasone did not affect the RANTES expression in allergic fibroblasts stimulated by TNF-alpha and non-allergic fibroblasts stimulated by IFN-gamma and TNF-alpha. CONCLUSION: This study shows different responses of the RANTES production in nasal fibroblasts to dexamethasone, perhaps reflecting heterogeneity of nasal fibroblasts.
