Electrospun Fibrous Silk Fibroin/Poly(L-Lactic Acid) Scaffold for Cartilage Tissue Engineering.
10.1007/s13770-016-9099-9
- Author:
Weiwei LIU
1
;
Zhengqiang LI
;
Lu ZHENG
;
Xiaoyan ZHANG
;
Peng LIU
;
Ting YANG
;
Bing HAN
Author Information
1. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China. hbing@jlu.edu.cn
- Publication Type:Original Article
- Keywords:
Electrospinning;
Silk fibroin;
Poly(L-lactic acid);
Cartilage tissue engineering;
Scaffold
- MeSH:
Cartilage*;
Cartilage, Articular;
Chondrocytes;
Extracellular Matrix;
Fibroins;
In Vitro Techniques;
Silk*;
Tensile Strength;
Tissue Engineering*
- From:
Tissue Engineering and Regenerative Medicine
2016;13(5):516-526
- CountryRepublic of Korea
- Language:English
-
Abstract:
For successful tissue engineering of articular cartilage, a scaffold with mechanical properties that match those of natural cartilage as closely as possible is needed. In the present study, we prepared a fibrous silk fibroin (SF)/poly(L-lactic acid) (PLLA) scaffold via electrospinning and investigated the morphological, mechanical, and degradation properties of the scaffolds fabricated using different electrospinning conditions, including collection distance, working voltage, and the SF:PLLA mass ratio. In addition, in vitro cell-scaffold interactions were evaluated in terms of chondrocyte adhesion to the scaffolds as well as the cytotoxicity and cytocompatibility of the scaffolds. The optimum electrospinning conditions for generating a fibrous SF/PLLA scaffold with the best surface morphology (ordered alignment and suitable diameter) and tensile strength (~1.5 MPa) were a collection distance of 20 cm, a working voltage of 15 kV, and a SF:PLLA mass ratio of S50P50. The degradation rate of the SF/PLLA scaffolds was found to be determined by the SF:PLLA mass ratio, and it could be increased by reducing the PLLA proportion. Furthermore, chondrocytes spread well on the fibrous SF/PLLA scaffolds and secreted extracellular matrix, indicating good adhesion to the scaffold. The cytotoxicity of SF/PLLA scaffold extract to chondrocytes over 24 and 48 h in culture was low, indicating that the SF/PLLA scaffolds are biocompatible. Chondrocytes grew well on the SF/PLLA scaffold after 1, 3, 5, and 7 days of direct contact, indicating the good cytocompatibility of the scaffold. These results demonstrate that the fibrous SF/PLLA scaffold represents a promising composite material for use in cartilage tissue engineering.
