Immunohistochemical Study on the TfBP Expression in the Embryonic Chick Cerebellum.
- Author:
Sang Woo OH
1
;
Je Hoon SEO
;
Sang Wook PARK
;
Dong Woon KIM
;
Cheol LEE
;
Eun Jung ROH
;
Gye Sun JEON
;
Tae Cheon KANG
;
Kyung Hoon LEE
;
Sa Sun CHO
Author Information
1. Department of Family Medicine, Seoul National University Hospital, Korea.
- Publication Type:Original Article
- Keywords:
Transferrin binding protein;
Oligodendrocyte;
Chick Embryo;
Cerebellum
- MeSH:
Animals;
Carrier Proteins;
Cell Movement;
Central Nervous System;
Cerebellum*;
Chick Embryo;
Embryonic Development;
Female;
Oligodendroglia;
Pregnancy;
Transferrin
- From:Korean Journal of Anatomy
2001;34(3):253-260
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We have previously demonstrated that transferrin binding protein (TfBP) is a reliable marker for mature oligoden-drocytes (OLGs) in the avian central nervous system (CNS). Unlike mammalian CNS in which OLGs are generated largely postnatally, avian OLGs are differentiated during embryonic development of CNS. In this study, several aspects of TfBP(+/-) OLG development were immunohistochemically examined in the embryonic chick cerebellum : (1) change in shapes of immature cells with respect to time and to location within the cerebellum, (2) possible sites of origin, and (3) pathways of precursor cell migration. Our results indicate that TfBP expression gradually increases and extends from the deep portion of the white matter to gray matter with proportion to progress of cerebellar development. A few TfBP? cells were first observed in the deep portion of the cerebellum at E9. At E13, TfBP(+/-) cells were distributed evenly within the white matter. At E17, many TfBP(+/-) OLGs were located at granular layer and at the near place of Purkinje cell layer. At E20, a large number of TfBP cells appeared at the granular layer with a few in the molecular layer. Our data demonstrated distinct patterns of morphology and location of TfBP(+/-) OLGs in the cerebellum during development and suggest a role of TfBP in OLG development.
