Positive Selection of Transgenic CD4 T Cells in the Restricted Peptide Environment.
- Author:
Dong Sup LEE
1
;
Wang Jae LEE
Author Information
1. Department of Anatomy, Seoul National University, College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Mouse;
CD4 T cell;
Restricted peptide
- MeSH:
Animals;
Bone Marrow;
Chimera;
Mice;
Mice, Transgenic;
Peptides;
Phenotype;
T-Lymphocytes*;
Thymocytes;
Thymus Gland
- From:Korean Journal of Anatomy
1999;32(5):727-733
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to investigate the role of H2-M molecule on class II molecule other than H2-Ab we made H2-M deficient H2-E transgenic mice and to see the peptide diversity on H2-E molecule in these mice, we contructed AND --> H2-M deficient H2-E transgenic bone marrow chimeras. The results were as followings. 1. The phenotype of thymus of H2-M deficient H2-E transgenic mice showed similar CD4 and CD8 single positive thymocyte amount as normal wild type B6 mice with CD4/CD8 ratio around 2. The percentage of double positive cells were about 85%. 2. In the periphery, H2-M deficient H2-E transgenic mice also showed similar amount of CD4 and CD8 cells as normal wild type B6 mice. The CD4/CD8 ratio was 1.5~2. 3. The development of transgenic CD4 cells from AND --> H2-M+/- H2-E transgenic bone marrow chimera was similar to wild type AND transgenic mice. 4. The development of transgenic CD4 cells from AND --> H2-M deficient H2-E transgenic bone marrow chimera was reduced compared to wild type AND transgenic mice or AND --> H2-M+/- H2-E transgenic bone marrow chimeras. But the amount of CD4 cells developed in these chimeras were significant and H2-E molecule could bind some self peptides in the absence of H2-M molecules and support the CD4 cell development in these chimeras.
