Screening of Interacting Proteins with PV.1 as Downstream Factors of BMP Signal.
- Author:
Yoo Seok HWANG
1
;
Jeong Pil CHAE
;
Dong Sun KIM
;
Kwon Moo PARK
;
Yong Chul BAE
;
Mae Ja PARK
Author Information
1. National Institute of Health, United States of America.
- Publication Type:Original Article
- Keywords:
BMP signal transduction;
PV.1 protein;
Xvent-2 protein;
Xclaudin-6 protein;
Yeast two-hybrid;
Xenopus laevis
- MeSH:
Axis, Cervical Vertebra;
Mass Screening*;
Mesoderm;
Transcription Factors;
Xenopus laevis;
Yeasts
- From:Korean Journal of Anatomy
2007;40(3):203-210
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Homeodomain transcription factors functioning downstream of BMP ventral pathway have been reported to share similar domain of roles in mesoderm patterning along the dorsal-ventral axis. To elucidate the differential role of PV.1 in the aspect of relationship between dorsal and ventral region, we tried to screen PV.1- interacting proteins. Twenty-four PV.1-interacting proteins were identified by yeast two-hybrid screening. Xvent-2 and Xclaudin-6 among these, went under domain study. The C-terminus of PV.1, more specifically 197-241 region was found to interact with Xclaudin-6. Meanwhile Xvent-2 has mild affinity to overall C-terminal region of PV.1. At the same time it was found that Xvent-2 homodimerizes and also binds to Xclaudin-6.
