Localization of Heat Shock Protein 72 in Potassium-deprivated Rat Kidney.
- Author:
Su Youn LEE
1
;
Myung Sun KIM
;
Ji Hyun SONG
;
Ju Young JUNG
;
Ki Hwan HAN
Author Information
1. Department of Anatomy, School of Medicine, Ewha Womans University, Seoul, Korea. khhan@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Potassium;
Kidney;
HSP72
- MeSH:
Animals;
Blotting, Western;
Cell Survival;
Diet;
Down-Regulation;
Epithelial Cells;
Heat-Shock Proteins*;
Hot Temperature*;
HSP72 Heat-Shock Proteins*;
Humans;
Immunohistochemistry;
Kidney*;
Male;
Osmolar Concentration;
Perfusion;
Potassium;
Rats*;
Rats, Sprague-Dawley
- From:Korean Journal of Anatomy
2007;40(3):243-249
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Heat shock protein 72 (HSP72) appears to play an important role in cell survival in the hypertonic conditions of the renal medulla. The purpose of this study was to examine the effect of potassium deprivation on renal HSP72 expression. Male Sprague-Dawley rats were fed potassium deficient diet for 2 weeks. Kidney tissues were preserved by in vivo perfusion with paraformaldehyde-lysine-periodate (PLP) and processed for Western blot analysis and immunocytochemistry. Serum potassium concentration and urine osmolality decreased in potassium deprivated animals. In control kidneys, HSP72 immunostaining was observed mainly in the inner medulla in almost all cells including the inner medullary collecting duct and papillary surface epithelial cells. In potassium deprivated kidneys, HSP72 expression decreased dramatically in the inner medulla. However, strong HSP72 immunostaining remained in some inner medullary collecting duct and papillary surface epithelial cell. These results demonstrated that potassium deprivation induced down regulation of HSP72 in the renal medulla, at least in part, through cell-specific manner.
