Effect of low-level laser therapy on bisphosphonate-treated osteoblasts.
10.1186/s40902-016-0095-8
- Author:
Sang Hun SHIN
1
;
Ki Hyun KIM
;
Na Rae CHOI
;
In Ryoung KIM
;
Bong Soo PARK
;
Yong Deok KIM
;
Uk Kyu KIM
;
Cheol Hun KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Beomeo, Mulgeum, Yangsan 626-770, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Bisphosphonate;
Osteoblasts;
Low-level laser therapy (LLLT);
BRONJ
- MeSH:
Alendronate;
Bone Remodeling;
Cell Culture Techniques;
Cell Line;
Cytokines;
Humans;
Low-Level Light Therapy*;
Macrophage Colony-Stimulating Factor;
Osteoblasts*;
Osteoprotegerin;
Seoul
- From:Maxillofacial Plastic and Reconstructive Surgery
2016;38(11):48-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: This study investigates the effect of alendronate-treated osteoblasts, as well as the effect of low-level laser therapy (LLLT) on the alendronate-treated osteoblasts. Bisphosphonate decreases the osteoblastic activity. Various treatment modalities are used to enhance the bisphosphonate-treated osteoblasts; however, there were no cell culture studies conducted using a low-level laser. METHODS: Human fetal osteoblastic (hFOB 1.19) cells were treated with 50 μM alendronate. Then, they were irradiated with a 1.2 J/cm² low-level Ga-Al-As laser (λ = 808 ± 3 nm, 80 mW, and 80 mA; spot size, 1 cm²; NDLux, Seoul, Korea). The cell survivability was measured with the MTT assay. The three cytokines of osteoblasts, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) were analyzed. RESULTS: In the cells treated with alendronate at concentrations of 50 μM and higher, cell survivability significantly decreased after 48 h (p < 0.05). After the applications of low-level laser on alendronate-treated cells, cell survivability significantly increased at 72 h (p < 0.05). The expressions of OPG, RANKL, and M-CSF have decreased via the alendronate. The RANKL and M-CSF expressions have increased, but the OPG was not significantly affected by the LLLT. CONCLUSIONS: The LLLT does not affect the OPG expression in the hFOB cell line, but it may increase the RANKL and M-CSF expressions, thereby resulting in positive effects on osteoclastogenesis and bone remodeling.
