Insights into the Therapeutic Potential of Heparinized Collagen Scaffolds Loading Human Umbilical Cord Mesenchymal Stem Cells and Nerve Growth Factor for the Repair of Recurrent Laryngeal Nerve Injury.
10.1007/s13770-017-0032-7
- Author:
Yongqin PAN
1
;
Genlong JIAO
;
Jingge YANG
;
Rui GUO
;
Jinyi LI
;
Cunchuan WANG
Author Information
1. Department of General Surgery, First Affiliated Hospital of Jinan University, No. 613 West Huangpu Avenue, Guangzhou 510630, People's Republic of China. tlijy@jnu.edu.cn, twcc@jnu.edu.cn
- Publication Type:Original Article
- Keywords:
Recurrent laryngeal nerve injury;
Human umbilical cord mesenchymal stem cells;
Nerve growth factor;
Collagen scaffolds;
Heparin
- MeSH:
Collagen*;
Dyspnea;
Electrophysiology;
Heparin*;
Hoarseness;
Humans*;
In Vitro Techniques;
Intermediate Filaments;
Mesenchymal Stromal Cells*;
Nerve Growth Factor*;
Paralysis;
Porosity;
Recurrent Laryngeal Nerve Injuries*;
Recurrent Laryngeal Nerve*;
Spectroscopy, Fourier Transform Infrared;
Umbilical Cord*;
Vocal Cords
- From:
Tissue Engineering and Regenerative Medicine
2017;14(3):317-326
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recurrent laryngeal nerve (RLN) injury can result in unilateral or bilateral vocal cords paralysis, thereby causing a series of complications, such as hoarseness and dyspnea. However, the repair of RLN remains a great challenge in current medicine. This study aimed to develop human umbilical mesenchymal stem cells (HuMSCs) and nerve growth factor (NGF)-loaded heparinized collagen scaffolds (HuMSCs/NGF HC-scaffolds) and evaluate their potential in the repair of RLN injury. HuMSCs/NGF HC-scaffolds were prepared through incorporating HuMSCs and NGF into heparinized collagen scaffolds that were prefabricated by freeze-drying in a template. The resulting scaffolds were characterized by FTIR, SEM, porosity, degradation in vitro, NGF release in vitro and bioactivity. A rabbit RLN injury model was constructed to appraise the performance of HuMSCs/NGF HC-scaffolds for nerve injury repair. Electrophysiology, histomorphology and diagnostic proteins expression for treated nerves were checked after application of various scaffolds. The results showed that the composite scaffolds with HuMSCs and NGF were rather helpful for the repair of broken RLN. The RLN treated with HuMSCs/NGF HC-scaffolds for 8 weeks produced a relatively normal electromyogram, and the levels of calcium-binding protein S100, neurofilament and AchE pertinent to nerve were found to be close to the normal ones but higher than those resulted from other scaffolds. Taken together, HuMSCs/NGF HC-scaffolds exhibited a high score on the nerve injury repair and may be valuable for the remedy of RLN injury.
