Stimulation of bone formation by direct electrical current in an orthopedically expanded suture in the rat.
10.4041/kjod.2010.40.2.106
- Author:
Tancan UYSAL
1
;
Mihri AMASYALI
;
Huseyin OLMEZ
;
Yildirim KARSLIOGLU
;
Omer GUNHAN
Author Information
1. Department of Orthodontics, Faculty of Dentistry, Erciyes University, Kayseri Turkey, and Visiting Professor, King Saud University, Riyadh, Saudi Arabia. tancanuysal@yahoo.com
- Publication Type:Original Article
- Keywords:
Electric stimulation;
Maxillary expansion;
Image analysis;
Rats
- MeSH:
Animals;
Bone Regeneration;
Electric Stimulation;
Electrodes;
Humans;
Incisor;
Male;
Osteogenesis;
Palatal Expansion Technique;
Rats;
Retention (Psychology);
Sutures
- From:Korean Journal of Orthodontics
2010;40(2):106-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this experimental study was to evaluate the effects of direct electrical current stimulation (DECS) on bone regeneration in response to an expansion of the inter-premaxillary suture in the rat. METHODS: Sixteen 50 - 60 days old Wistar male rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30-gram of force was applied to the maxillary incisors with helical-spring. In the experimental group, two metallic-screws were placed at lateral parts of the maxillary segments. Electrodes were connected to the screws. The device was activated with current adjustment to measure 10 microA continuously and the current was monitored daily during the expansion and early-retention phase. Bone regeneration in the sutural area was histomorphometrically evaluated including new-bone area (micrometer2), bone perimeter (micrometer), feret's diameter (micrometer) and newly formed bone (%) parameters. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p < 0.05 level. RESULTS: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. New bone area (p = 0.002), bone perimeter (p = 0.004), feret's diameter (p = 0.002) and newly formed bone percentage (p = 0.002) measurements were significantly higher in the experimental group than the control group. Bone histomorphometric measurements revealed that bone architecture in the DECS group was improved. CONCLUSIONS: The application of DECS to an orthopedically expanded inter-premaxillary suture area during the early retention phase stimulated the formation of new bone.
