Immunohistochemical Localization of Choline Acetyltransferase in External Branch of Superior Laryngeal Nerve in Cat.
- Author:
Young Mo KIM
1
;
Chang Joon HAN
;
Jung Il CHO
;
Young Seok CHUNG
;
Tae Sook HWANG
;
Dae Joong KIM
Author Information
1. Department of Otorhinolaryngology, College of Medicine, Inha University, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Superior laryngeal nerve;
Sensory nerve fiber;
Choline acetyltransferase;
Immunohistochemistry
- MeSH:
Adult;
Animals;
Cats*;
Choline O-Acetyltransferase*;
Choline*;
Humans;
Hypoglossal Nerve;
Immunohistochemistry;
Laryngeal Nerves*;
Motor Neurons;
Nerve Fibers
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1997;40(3):409-416
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Existence of the external branch of the superior laryngeal nerve containing afferent fibers is much of concern. In this study, it is the aim to identify the component of fibers by immunohistochemistry using choline acetyltransferase, the specific marker of the cholinergic motor neuron. Both side of external branch of superior laryngeal nerve were excised from 4 adult cats, and also obtained the great auricular nerve and the hypoglossal nerve for sensory control and motor control specimen, respectively. There were no fibers with immune reaction in the great auricular nerve while all of the fibers in the hypoglossal nerve were strongly stained with rabbit anti-choline acetyltransferase antibody. In the external branch of the superior laryngeal nerve, 12.7% of the fibers were not stained with immune reaction product. They are smaller than stained nerve fibers in cross sectioned diameter and peripherally located in the same fascicle. The identification of unstained fiber group in the external branch of the superior laryngeal nerve suggest that it is a mixed nerve and not purely motor, possibly containing the sensory nerve fiber.
