Effect of Retinoic Acid on the Expression of PDGF Receptors During Palatogenesis.
- Author:
Yong Jin JANG
1
;
Byung Yong PARK
;
Mong Yong JUNG
;
Ki Tak KWON
;
Jong Youn KIM
;
Yeon Ho JEONG
;
Young Hoon LEE
;
Byung Keon PARK
Author Information
1. Department of Oral Anatomy, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University, Korea. bkpark@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Palate development;
PDGFR-alpha;
PDGFR-beta;
Retinoic acid
- MeSH:
Animals;
Cleft Lip;
Cleft Palate;
Immunohistochemistry;
Intercellular Signaling Peptides and Proteins;
Mice;
Palate;
Receptors, Platelet-Derived Growth Factor*;
Tretinoin*
- From:Korean Journal of Anatomy
2006;39(5):383-392
- CountryRepublic of Korea
- Language:English
-
Abstract:
Although cleft lip and palate are one of the most common craniofacial malformation, little is still known about the mechanism of the palate formation. Retinoic acid (RA) is known a teratogen, and cleft palate is induced by retinoic acid administration in the secondary palate formation stage. Many growth factors and their receptors are involved in the formation of the secondary palate. Here, we investigated the expression of PDGFR-alpha, and PDGFR-beta during palatogenesis after retinoid acid administration in mice by RT-PCR and immunohistochemistry. At E15.5, the opposing palatal shelves fused with one another in the control group, but the palatal shelves were not elevated and cleft palate was induced in the RA-treated group. In RT-PCR, PDGFR-beta was downregulated during palatogenesis after RA administration. In immunohistochemical experiment, PDGFR-alpha and PDGFR-beta were reduced in RA-induced group. Taken together, we suggest that PDGF receptors may be molecules involved in palate formation.
