Mutations of the p53 tumor suppressor gene in rheumatoid arthritis synovium.
- Author:
Chang Whan HAN
1
;
Hyung Kwan KIM
;
Weon You KIM
;
Kwang Won LEE
;
Eui Yong OHM
;
Jung Man KIM
Author Information
1. Department of Orthopaedic Surgery, Taejon St. Mary's Hospital, University of Catholic, Korea.
- Publication Type:Original Article
- Keywords:
Rheumatoid Arthritis;
p53;
Direct sequence;
Immunohistochemistry
- MeSH:
Amino Acid Substitution;
Arthritis, Rheumatoid*;
Base Sequence;
Biopsy;
Codon;
DNA;
Genes, Suppressor;
Genes, Tumor Suppressor*;
Humans;
Immunohistochemistry;
Membranes;
Osteoarthritis;
Synovial Membrane*
- From:The Journal of the Korean Orthopaedic Association
2001;36(1):9-16
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To analyse the expression of the p53 tumour suppressor gene in the synovial tissue from rheumatoid patients. Material and Methods : Synovial membranes were obtained from 13 patients diagnosed as having RA, and 9 osteoarthritis (OA) patients. We studied p53 expression by immunohistochemical analysis and p53 DNA sequence using direct DNA preparation method. RESULT: In immunohistological studies, the Do-1 monoclonal antibody stained at 6 specimens out of the 13 rheumatoid arthritis tissue biopsies analysed. There was no p53 mutation in osteoarthritis samples, but there were 4 p53 mutations from the 13 rheumatoid arthritis samples. p53 mutations were found at the codon 177 (CTG to CTA, GA), 277 (TGT to TGC, TC), and, two patients at the codon 237 (CAT to TAT, CT). CONCLUSION: The predicted amino acid substitutions in p53 were similar to those commonly observed in a variety of tumors and might influence growth and survival of rheumatoid synoviocytes.
