The study of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve.
- Author:
Kwang Suk LEE
1
;
Jae Hak SHIM
;
Tae Ha KIM
;
Sung Dae JU
Author Information
1. Department of Orthopedic Surgery, Korea University, Seoul, Korea .
- Publication Type:Original Article
- Keywords:
Defect of nerve;
inside-out vein graft;
nerve growth factor
- MeSH:
Animals;
Axons;
Electromyography;
Microscopy;
Microscopy, Electron, Transmission;
Nerve Growth Factor*;
Nerve Regeneration*;
Rats;
Rats, Sprague-Dawley;
Regeneration;
Sciatic Nerve*;
Transplants*;
Veins*
- From:The Journal of the Korean Orthopaedic Association
2001;36(1):17-24
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To compare the effect of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve of rat. MATERIALS AND METHODS: Eighty Sprague-Dawley rats were divided into five experimental groups: orthotopic nerve graft as control group, inside-in vein graft with normal saline infiltration, inside-out vein graft with normal saline infiltration, inside-in vein graft with nerve growth factor infiltration and inside-out vein graft with nerve growth factor infiltration group. Animals of each group were sacrificed after electromyography at 2, 4, 8 and 12 weeks after operation. Also the light microscopy and transmission electron microscopy were performed to observe histologic change of grafted sites. RESULTS: This study demonstrates that autogenous vein graft serves as a conduit for nerve regeneration, and inside-out vein graft technique and nerve growth factor induce faster and more numerous axonal regeneration and earlier recovery of muscle power. CONCLUSION: Inside-out vein graft with nerve growth factor infiltration is supposed to be a promising technique to take the place of autogenous nerve graft.
