Multidrug resistance-associated protein (MRP) and multidrug resistance (MDR1) gene expression in osteosarcoma and their prognostic significance.
- Author:
Hong Geun JUNG
1
;
Sang Hoon LEE
;
Han Soo KIM
Author Information
1. Department of Orthopaedic Surgery, Seoul National University College of Medicine.
- Publication Type:Original Article
- Keywords:
Osteosarcoma;
Multidrug resistance-associated protein;
Multidrug resistance gene;
Chemotherapy response
- MeSH:
Drug Resistance, Multiple*;
Drug Therapy;
Gene Expression*;
Genes, Essential;
Genes, MDR;
Multidrug Resistance-Associated Proteins*;
Multivariate Analysis;
Osteosarcoma*;
RNA
- From:The Journal of the Korean Orthopaedic Association
2001;36(1):1-8
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study is to evaluate the correlations between the multidrug resistance-associated protein (MRP) and multidrug resistance (MDR1) gene in osteosarcoma, and their prognostic significances by RT-PCR method. MATERIALS AND METHODS: We isolated RNA from 34 fresh deep-frozen primary osteosarcoma tissue specimens and evaluated the expression level of MRP and MDR1 m-RNA using RT-PCR method. GAPDH was used as the housekeeping gene for the experiment. RESULTS: The average MRP/GAPDH m-RNA ratio was 0.550 and the average MDR1/GAPDH m-RNA ratio was 0.419. There were moderate degree of correlations between MRP/GAPDH m-RNA and MDR1/GAPDH m-RNA ratio (p<0.05). There was significant correlations between the chemotherapy response and the MDR1/GAPDH m-RNA ratio (p<0.05), but not with the MRP/GAPDH ratio. Multivariate analysis for the correlation between the MRP and MDR1 gene expression and the chemotherapy response revealed that only the MDR1/GAPDH m-RNA ratio was significantly related to it (p<0.05). CONCLUSION: There was a moderate degree of correlation between the expressions of MRP and MDR1 genes. However, the chemotherapy response showed significant correlations with the expression of MDR1 gene, but not with MRP gene.
