The expression of type I collagen in periodontal tissue during the experimental movement of rat incisors.
- Author:
Sang Cheol KIM
1
;
In Seop JEON
Author Information
1. Department of Orthodontics, College of Dentistry, Wonkwang University, Korea.
- Publication Type:Original Article
- Keywords:
orthodontic force;
tooth movement;
bone remodeling;
type I collagen
- MeSH:
Animals;
Bone Matrix;
Bone Remodeling;
Cell Differentiation;
Collagen Type I*;
Connective Tissue;
Dental Cementum;
Epithelium;
Incisor*;
Osteoblasts;
Periodontal Ligament;
Rats*;
Rats, Sprague-Dawley;
Streptavidin;
Sutures;
Tooth Movement
- From:Korean Journal of Orthodontics
1996;26(4):455-466
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was designed to evaluate the expression of type I collagen in periodontal tissue during the experimental movement of rat incisors. Twenty-one Sprague-Dawley rats were divided into a control group(3 rats), and experimental groups(18 rats) where a force(75g) from helical springs across the maxillary incisors was applied. Experimental groups were sacrificed at 12 hours, 1, 4, 7, 14 and 28 days after force application, respectively. And tissue slides of control and experimental groups were studied histologically and immunohistochemically by LSAB(Labelled streptavidine Biotin) immunohistochemical staining for type I collagen. The results were as follows: 1. Until 28-day after force application, periodontal fibers were strectched on the tension side, and compressed in pressure side, and the arrangement of periodontal fibers was not recovered by that time. 2. The degree of type I collagen expression in control group was rare in the oral epithelium, predentin, pulp and periodontal ligament, but was mildly positive in osteoblasts, acellular cementum, cementoblasts, intermaxillary suture. 3. At acellular cementum of experimental group, the expression of type I collagen was moderate in 1-day and severe in 7-day, which was maintained until 28-day. 4. Type I collagen was observed in the newly formed fibrous connective tissue and osteoblasts at intermaxillary suture, moderately in 1-day, and severely in 14-day. 5. The tension side of periodontal ligament showed a more positive expression of type I collagen than the pressure side in 4-day. The degree was highest in 7-day and was not differentiated between sides in 14-day. 6. In the side wall of bone matrix on which osteoblasts were attached, type I collagen was expressed severely, especially in 7-day. From the above findings, we could suggest that bone remodeling in tooth movement be intimately related to the cell differentiation and the resulting formation of type I collagen.
