A change of nitric oxide in rat DRG following Freund's Complete Adjuvant induced inflammtory pain.
- Author:
Seung Jun HWANG
1
;
Jong Hwan LEE
;
Tai Jin CHUNG
;
Hea Nam HONG
;
Joong Woo LEEM
;
Jai Hyun HWANG
;
Yoon CHOI
Author Information
1. Department of Anatomy, University of Ulsan, College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
FCA;
Inflammation;
Dorsal root ganglia;
Pain;
NOS;
NADPH;
L-NAME
- MeSH:
Animals;
Diagnosis-Related Groups*;
Extremities;
Ganglia, Spinal;
Hindlimb;
Humans;
Inflammation;
NAD;
NADP;
Neurons;
NG-Nitroarginine Methyl Ester;
Nitric Oxide Synthase;
Nitric Oxide*;
Pain Perception;
Rats*
- From:Korean Journal of Anatomy
2000;33(2):135-142
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It has been reported that injection of Freund's complete adjuvant (FCA) into the hindpaw of a rat induces inflammatory responses with accompanying pain behaviors. Signs of pain behaviors observed in FCA-injected animals were reported to be similar to symptoms seen in patients with inflammatory pain. The nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) is a selective histochemical marker for the nitric oxide synthesizing enzyme, nitric oxide synthase (NOS). N (G)-nitro-L-arginine methyl ester (L-NAME) is a NOS inhibitor. In the present study, we examined if inflammaory pain causes increases in NADPH-diaphorase reactivities in neurons of the dorsal root ganglia (DRG). The results were as follows; 1. FCA-induced inflammation on a limb increased staining density (SD) of NADPH-d positive neurons in the ipsilateral side DRG. 2. Pretreatment of L-NAME did not changed SD of NADPH-d positive neurons on the inflammation of contralateral side DRG 3. Posttreatment of L-NAME decreased the inflammation induced SD of NADPH-d positive neurons. 4. n-NOS immunoreactivity did not match NADPH-d histochemical study, implying the constant level of enzyme itself. Inflammation pain on a hindlimb increased staining density of NADPH-diaphorase positive neuron in the DRG, which was decreased by L-NAME. L-NAME also decreased pain perception. This suggests a role of NO in the pain perception and/or modulation at the level of DRG.
