Detection of Epstein-Barr Virus DNA in Oropharynx by Polymerase Chain Reaction.
- Author:
Nam Yong DOH
1
;
Wan Soo KIM
;
Sung Hyun KIM
;
Jun Han LEE
;
Do Yong LEE
;
Bong Nam CHOI
Author Information
1. Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Chosun University, Kwang-Ju, Korea.
- Publication Type:Original Article
- Keywords:
Epstein-barr virus(EBV);
Polymerase chain reaction(PCR);
Throat washing
- MeSH:
Burkitt Lymphoma;
DNA*;
Head;
Herpesvirus 4, Human*;
Hodgkin Disease;
Humans;
Infectious Mononucleosis;
Neck;
Oropharynx*;
Pharynx;
Polymerase Chain Reaction*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1998;41(1):73-76
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The Epstein-Barr virus (EBV) is a human herpes virus which causes infectious mononucleosis and is associated with such human cancers as Burkitt's Lymphoma, Hodgkin's disease, non-Hodgkin's disease, and nasopharyngeal carcinoma. However, the role of EBV as a causative factor in other head & neck tumors is not fully elucidated. This study analyzed the detection rate of Epstein-Barr virus (EBV) in the throat washing samples from pateints with head and neck tumors and non-malignant diseases, as well as from normal subjects. MATERIALS & METHODS: The throat washing samples were collected from 49 patients of head & neck tumors, 52 patients of non-malignant diseases (pharyngitis and tonsillitis) and 24 normal subjects. The EBV DNA was detected by using the EBV (BNRF1)-specific primers & Polymerase Chain Reaction (PCR). RESULT: The EBV DNA was detected in 25 (51%) of the 49 throat washing samples from patients with tumors, as well as 3 (60%) of 5 samples from patients with nasopharyngeal carcinoma, and 22 (50%) of 44 samples from patients with head and neck tumors. The throat wasing samples from 52 patients with non-malignant diseases and 24 samples from healthy subjects were also examined. EBV was detected in 17 (32.7%) of 52 patients with non-malignant diseases and 3 (12.5%) of 24 samples from healthy subjects. CONCLUSION: These data indicate that the EBV may be related to non-malignant diseases and head and neck tumor.
