Performance of LIFECODES HLA-DQB1 Typing Kit Using Luminex Platform in Koreans.
10.3343/alm.2015.35.1.123
- Author:
Eun Youn ROH
1
;
Ji Won IN
;
Sue SHIN
;
Jong Hyun YOON
;
Kyoung Un PARK
;
Eun Young SONG
Author Information
1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. eysong1@snu.ac.kr
- Publication Type:Brief Communication ; Evaluation Studies
- Keywords:
HLA-DQB1;
PCR-SSO;
Luminex;
Korean;
Organ transplantation
- MeSH:
Alleles;
DNA Primers/metabolism;
Gene Frequency;
Genotype;
HLA-DQ beta-Chains/*genetics/metabolism;
Histocompatibility Testing/*standards;
Humans;
Polymerase Chain Reaction;
Reagent Kits, Diagnostic/*standards;
Republic of Korea
- From:Annals of Laboratory Medicine
2015;35(1):123-127
- CountryRepublic of Korea
- Language:English
-
Abstract:
Intermediate-resolution HLA-DQ typing has gained importance in organ transplantation recently. We evaluated the performance of the LIFECODES HLA-DQB1 typing kit (Immucor, USA) using sequence-specific oligonucleotide (SSO) probe and Luminex platform (Luminex Corp., USA) on 100 samples tested by sequence-based typing (SBT) using the AlleleSEQR HLA-DQB1 kit (Abbott Molecular, USA) in Korean individuals. No sample showed ambiguity in the assignment of 4-digit HLA-DQB1 allele with the LIFECODES HLA-DQB1 SSO typing kit, and the results were fully concordant with those of high-resolution typing of AlleleSEQR HLA-DQB1 SBT up to 4-digit level. Three samples required adjustment of false reactions (3/100, 3.0%): two samples with DQB1*03:03/*06:01 showed false-positive result in probe 253, and 1 sample with DQB1*04:02/*05:02 showed false-negative result in probe 217. We tested an additional sample with DQB1*03:03/*06:01, which showed same false-positivity in probe 253 and 2 samples with DQB1*04:02/*05:02, which showed no false reaction. The false reactions did not result in ambiguity or change in the HLA allele assignment. We could assign HLA-DQB1 alleles to 4 digit-level without ambiguity, with 100% concordance with the SBT results. Thus, LIFECODES HLA-DQB1 SSO typing kit showed good performance for intermediate-resolution HLA-DQB1 typing in clinical laboratory for organ transplantation in Koreans.
