Effect of Growth Factors on Hearing Restoration in Deafened Animal Model.
- Author:
Ji Hong KIM
1
;
Shi Nae PARK
;
Sang W YEO
;
Young Ha KIM
;
Sun Young JIN
;
Jong Hoon KIM
;
Sang Jae CHO
;
Jung Hyun KIM
;
Mi Yung CHOI
;
Kyoung Ho PARK
Author Information
1. Department of Otolaryngology-HNS, College of Medicine, The Catholic University of Korea, Seoul, Korea. khpent@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Growth factors;
Guinea pig;
Hearing gain
- MeSH:
Animals;
Brain-Derived Neurotrophic Factor;
Ear;
Ear, Inner;
Epidermal Growth Factor;
Ethacrynic Acid;
Glial Cell Line-Derived Neurotrophic Factor;
Guinea;
Guinea Pigs;
Hair;
Hearing;
Hearing Loss, Sensorineural;
Intercellular Signaling Peptides and Proteins;
Kanamycin;
Models, Animal;
Nerve Growth Factors;
Neurons;
Noise;
Reflex;
Spiral Ganglion
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2008;51(12):1093-1098
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Sensorineural hearing loss is caused by ototoxic drugs, radiation therapy, noise exposure and trauma, etc. They make irreversible changes in cochlear hair cells and degeneration of spiral ganglion neurons. It is known that neurotrophins and other growth factors have an important role in protectingcochlear hair cells and spiral ganglion neurons. We designed this study to analyze the effect of neurotrophins and growth factors delivered to the inner ear of deafened guinea pig. MATERIALS AND METHOD: Healthy 15 guinea pigs with normal Preyer's reflex were chosen, and were made deaf by infusion of kanamycin and ethacrynic acid. Myringotomy was done to both ears, normal saline injected into the left ear, and BDNF (brainderived neurotrophic factor), GDNF (glial cell derived neurotrophic factor), NT-3 (neurotrophin-3), IGF (insulin-like growth factor), EGF (epidermal growth factor), FGF (fibroblast growth factor) were injected in the right ear. RESULTS: Statistically significant hearing gain was obtained up to 35.00+/-13.78 dB in the group 1 (BDNF, GDNF, NT-3 treated group), and up to 34.0+/-5.47 dB in the group 2 (IGF treated group). However, no statistically significant hearing gain was observed in the group 3 (EGF, FGF treated group). CONCLUSION: We observed statistically important improvement of hearing threshold in the BDNF, GDNF, NT-3 treated group and IGF treated group.
