Genetic polymorphisms of arsenic methyltransferase(AS3MT)in 16 Chinese ethnic populations
- VernacularTitle:中国16个人群砷甲基转移酶AS3MT基因5'-UTR区VNTR多态性研究
- Author:
Song-bo, FU
;
Jie, WU
;
Feng, CHEN
;
Dian-jun, SUN
- Publication Type:Journal Article
- Keywords:
Genetic polymorphism;
Arsenic;
Aarsenic methyltransferase;
Population genetics
- From:Chinese Journal of Endemiology
2008;27(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and evaluate the polymorphism distribution of arsenic(+3 oxidation state)methyhransferase(AS3MT)5'-UTR VNTR in Chinese populations.Methods Genomic DNA was extracted from peripheral blood anti-coagulated with ACD of 1440 individuals in a standard phenol-chloroform protocol.The phenotypes of AS3MT 5'-UTR VNTR were determined by polymerase chain reaction(PCR)associated with agarose gel electrophoresis.Results Of the 1440 individuals,771(53.5%),426(29.6%),211(14.7%),16(1.1%)and 16(1.1%)were carriers of the V2/V3(AB/A2B),V3/V3(A2B/A2B),V2/V2(AB/AB),V2/V4(AB/A3B)and V3/V4(A2B/A3B)genotype,respectively.The AB(V2),A2B(V3)and A3B(V4)allele frequency was 41.9%,57.0%,1.1%respectively.The differences of AB(V2)and A2B(V3)allele frequency were all significant between the northern and southern populations respectively(χ2=23.39,χ2=33.28,P＜0.007).Conclusions In different regions the AB(V2)and A2B(V3)allele frequency is different,the AS3MT 5'-UTR VNTR polymorphism can be used to evaluate the susceptivity of arsenieosis.
