Expression of PURA gene and its protein in nephridial tissue of rats with fluorosis of coal-burning
10.3760/cma.j.issn.1000-4955.2010.02.006
- VernacularTitle:燃煤污染型氟中毒大鼠肾组织中PURA基因及其蛋白的表达
- Author:
Jia-qi, WANG
;
Ji-can, LIU
;
Yan-ni, YU
;
Chang-wu, WAN
- Publication Type:Journal Article
- Keywords:
Fluoride poisoning;
Kidney;
Immunohistochemistry;
PURA gene
- From:Chinese Journal of Endemiology
2010;29(2):135-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the meaning of PURA gene and its protein in nephridial tissue of the rats with endemic fluorosis of coal burning. Methods Thirty-six SD rats of 80 - 100 g, body weight were randomly divided into control group, low fluorosis group and high fluorosis group according to body weight, 12 in each group, the number of female and male in each group was the same respectively. The control group, Low fluorosis group and high fluorosis group rots were fed with 1.5,25.0,60.0 mg/kg fluoride content in feedstuff, to establish the animal model of fluorosis. Expressions of both mRNA and its protein of PURA gene in rat nephridium tissue, were determined by RT-PCR and immunohistochemistry after four-month experimental period. Results The expressions of PURA mRNA[(2.74± 1.06),(4.29 ± 2.11)] and its protein[ (28 827.91 ± 4801.94),(61 146.96 ± 4997.55)] in low fluorosis group and high fluorosis group was higher than that in the control group[ ( 1.13 ± 0.87), (7131.95 ± 1524.54), all P < 0.05]. And the expressions of PURA mRNA and protein in high fluorosis groups was higher than that in low fluorosis greup(all P < 0.05). Conclusion High fluoride can lead to the high expression of PURA gene mRNA and protein in the rat nephridium tissue exposed to sodium fluoride.
