Study on changes of TNF-α and IL-1 in spleen cells of mice induced by recombinant BCG-Sj26GST vaccine of schistosoma japonicum
- VernacularTitle:日本血吸虫重组BCG-Sj26GST疫苗诱导小鼠脾细胞TNF-α和IL-1变化的研究
- Author:
Wen-gui, LI
;
You-en, SHI
- Publication Type:Journal Article
- From:Chinese Journal of Endemiology
2001;20(3):172-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of recombinant BCG-Sj26GST vaccine of schistosoma japonicum of TNF-α and IL-1 produced by spleen cells of mice.Methods In experiment 1,BALB/C mice were immnized subcutaneously by 106 and 108 CFU vaccine respectively,challenged with Sj cercariae on 8 w of immunization and killed on 6 w of infection to separate spleens,PBS served as control;in experiment 2,after immunized subcutaneously and intravenously by 106 CFU vaccine respectively,4 mice were killed to separate sleens on 0,4,8,10,14 and 16 w of immunization,spleen cells were stimulated by Sj26 or mitogens,the level of TNF-α in supernatant of spleen cells were detected by ELISA,that of IL-1 by fibroblast proliferative response.Results Levels of TNF-α and IL-1 increased obviously by immunization with the vaccine against challenge with Sj cercariae;dynamic observation showed that TNF-α and IL-1 in the subcutaneous group reached the highest level on 4～8 w;that in the intravenous group on 16 w and 4～8 w respectively.Conclusions Recombinant BCG-Sj26GST vaccine of schistosoma japonicum may promote spleen cell of mice to secrete TNF-α and IL-1 early,these cytokines may play an important role in protective immunity against schistosomiasis.
