Effect of ascorbic acid combined with arsenic trioxide on apoptosis and differentiation in myelocytic leukemia cell lines
- VernacularTitle:抗坏血酸联合三氧化二砷对不同髓系白血病细胞凋亡与分化效应的差异化影响
- Author:
Sheng-jin, FAN
;
Li-min, LI
;
Jin, ZHOU
- Publication Type:Journal Article
- Keywords:
Arsenicals;
Ascorbic acid;
Apoptosi8;
Cell differentiation
- From:Chinese Journal of Endemiology
2008;27(5):495-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of arsenic trioxide(As2O3)combined with ascorbic acid(AA) on the apoptosis and differentiation of myelocytic leukaemia cells.Methods The acute promyelecytic leukaemia cell lines (NB4 and MR2)and erythroleukemia cell line(KS62)were cultured in vitro.Grouping wasbased on different concentration of As2O3(0,0.1,0.2,0.5,1.0 μmol/L),which WaS used a8 control groups.Then,AA(113.0μmol/)was added into each group.Cell morphologic changes of cell lines NB4,MR2 and K562 were observed under light microscope;The apoptosis symbols [Annexin V(+)/PI(-),Annexin V(+)/PI(+)]and differentiation symbols(CD11b and CD33)were detected by flow cytometry 96 hours later.Results
