Transfection of embryonic stem cells with green fluorescent protein gene and their differentiation into neural cells
- VernacularTitle:绿色荧光蛋白基因转染小鼠胚胎干细胞及向神经细胞的分化
- Author:
Zhi-yan, SHAN
;
Jing-ling, SHEN
;
Lei, LEI
;
Yan-ning, XU
;
Lian-hong, JIN
- Publication Type:Journal Article
- Keywords:
Embryonic stem cell;
Green fluorescent proteins;
Electroporation;
Cell differentiation
- From:Chinese Journal of Endemiology
2008;27(4):397-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish embryonic stem cells (ESC) that can express green fluorescent protein (CFP) and differentiate them into neurons. It would provide tagging neurons for clinical transplantation to cure neural system diseases. Methods ESC (R1) was transfeeted with a plasmid containing the GFP by electroporation. A transgeuic cell line was obtained after selection with G418. The ESCs were characterized by AKP staining. Monolayer differentiation method was used to induce neural differentiation derived from GFP-ESC and immunofluorescence method was used to identify Tuj1 positive cells. Results There was no significant difference(X2=3.14,P0.05) in transfect rates between liposome and electroporation (65% vs 79%). The AKP staining of GFP-ESC was positive. GFP-ESC could be differentiated into neural cells. Conclusions These results show that ESC expressing GFP has been estabhshed, which can be differemiated into neurons.
