Active effect of hemeoxygenase-1 expression on Chang liver cell induced by inorganic arsenic
10.3760/cma.j.issn.1000-4955.2009.01.003
- VernacularTitle:无机砷对Chang liver细胞血红素单加氧酶-1表达的活化作用
- Author:
Xin-yu, ZHANG
;
Bing, LI
;
Xin, LI
;
Bo, ZHU
;
Yong-yong, HOU
;
Peng, XUE
;
Gui-fan, SUN
- Publication Type:Journal Article
- Keywords:
Sodium arsenite;
Hemeoxygenase-1;
Chang liver cell
- From:Chinese Journal of Endemiology
2009;28(1):7-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether sodium arsenite(NaAsO2)can activate the expressions of hemeoxygenase-1(HO-1)of normal human liver cell line named Chang liver.Methods Chang liver cells were exposed to NaAsO2 at 10 μmoL/L,0(contml),2,6,12,24 h and at 0(control),5,10,25,50 μmol/L in 12 h, followed by the measuring of the expressions of HO-1 protein in ceUs with western blot.Results In 10 μmol/L groups Chang liver cells exposed for 6,12,24 h cultured in vitro,the expressions of HO-l protein(3.97±0.72, 12.92±2.98,23.29±3.82)was significantly higher than that of control(1.00±0.00),and compared with the control, the difference being statistically significant(F=85.83,P＜0.01;t=-9.42,-8.95,-13.83,respectively,P＜ 0.05 or＜0.01).In 12 h,5,10,25 and 50 μmol/L groups cultured in vitro,the expressions of HO-1(16.34±0.25, 7.75±0.39,7.93±0.14,12.48±0.35)was significantly higher than that of control(1.00±0.00).and compared with the control,the difference being statistically significant(F=85.83,P＜0.01;t=-36.25,-30.19,-86.40, -56.40,respectively,all P＜0.01).Conclusion Inorganic arsenic call induce the activation of HO-1,promote the expression of protein in a time-and dose-dependent manner.
